ASTM E2250-02

1.1 This method covers quantitative methods for the sampling and determination of Gram-negative bacterial endotoxin concentrations in water miscible metalworking fluids (MWF).

1.2 Users of this method should be familiar with the handling of MWF.

1.3 This method gives an estimate of the endotoxin concentration of the sampled MWF.

1.3.1 Used on site, this method gives an indication of changes in Gram-negative bacterial contamination in the MWF.

1.3.2 This method does not replace Practice E 2144.

1.4 This method seeks to minimize inter-laboratory variation but does not ensure uniformity of results.

1.5 This method is not intended to relate endotoxin concentration in MWF to health effects of inhaled endotoxin.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.



Keywords: endotoxin; endotoxin assay; endotoxin units (EU); fluorescence polarization; Gram-negative bacteria; MWF; ICS Number Code 13.060.70 (Examination of water for biological properties)


Note: This is a withdrawn item.